attune flow cytometer - An Overview
attune flow cytometer - An Overview
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Knowledge-enriched characterization of mobile populations. Morphological details from photos can add into the richness of flow cytometry knowledge. By way of example, the figure shows an in any other case standard apoptosis assay utilizing Annexin V and PI, introducing mobile imaging to characterize cells in Each individual populace to reveal morphologically distinct functions. These insights couldn't happen to be acquired from multiplex staining on your own.
As samples are obtained around the Attune CytPix Flow Cytometer, the substantial-velocity brightfield digital camera captures and shops visuals of detected activities in a rate as much as six,000 photos per 2nd, dependant upon the flow fee and impression dimension. For bigger flexibility, Attune Cytometric Software package permits you to alter the picture seize frequency as essential and select a particular gate for picture seize, if essential.
Merge acoustic focusing technology with higher-parameter analysis for adaptable spectral unmixing and traditional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating which has a higher-pace brightfield digital camera for simultaneous large throughput flow cytometry and higher resolution brightfield imaging.
"A picture is not really worthy of 1000 dots, but it truly helps to see visuals. It is excellent to see photographs that correlate with staining. By way of example, AnnexinV-favourable cells which have been morphologically distinct than healthful cells, or CD14+ monocytes which can be larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it's illuminating to view the amount of doublets slip by means of classic singlet gates, and fantastic to confirm the position of unforeseen double-favourable gatherings in my Investigation."
Pair your Attune flow cytometer with an autosampler for enhanced workflow effectiveness, and incorporate robotic integration for max throughput.
Steady image excellent even at significant throughput Acoustic focusing and also a higher-velocity digital camera Blend to graphic these T cells regularly at low or superior flow costs. Effortlessly adjust concentration and camera settings to fulfill experimental demands.
The Attune NxT cytometer would be the responsible workhorse instrument your lab can trust in for reliable and reliable overall performance. The Attune CytPix model includes a higher-velocity brightfield digital camera and AI-pushed picture Investigation software package, with modern updates like equipment Studying tools and an intuitive consumer interface, enabling Improved facts precision, precision, and more rapidly insights into sample biology.
Consumers can view occasion images in true-time to substantiate sample top quality, improve protocols, and history the morphology of each cell inhabitants determined for Examination. Imaging capture also benefits from acoustic concentrating, which helps to posture the cells to ensure that a pointy, centered picture is acquired.
AI-driven automated graphic Evaluation With all the Attune Cytometric Application interprets event features into distinct morphology parameters which can be coupled with common fluorescence and scatter parameters to reveal really reproducible inhabitants adjustments and special subsets.
Software Take note: Automated graphic analysis lowers consumer-to-person variability in flow cytometry gating procedures
Leverage the strength of Highly developed technological know-how and innovation to accelerate your discoveries. Believe in within the Attune Flow Cytometer loved ones to deliver the trustworthiness, pace, and precision that click here set your work aside.
Versatile payment system from Agilent Fiscal Alternatives assist you to obtain the important instrumentation you would like for instant venture, or to develop your lab.
Gating on these extended parameters helps you to swiftly and precisely recognize populations of fascination and confirm gating method with little if any guide assessment. The Attune application gives usage of condition-of-the-art device Understanding Examination instruments within an expertly built user interface, rendering it exceptionally user friendly.
Since the mobile line experienced previously been analyzed over the Attune CytPix Flow Cytometer, the researchers went again to the images and were being in a position to doc the microbial an infection not less than 5 days before.
The Attune CytPix Flow Cytometer makes it possible for customers to visualise samples throughout acquisition, confirm the accuracy of gating strategies, and guidance outcomes with reliable documentation for publication applications.
What gave the impression to be a homogeneous inhabitants is in fact a lot more varied—an insight that should be regarded as when interpreting effects.